Extracellular protein degradation on demand

نویسندگان

چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Phenotypes on demand via switchable target protein degradation in multicellular organisms

Phenotypes on-demand generated by controlling activation and accumulation of proteins of interest are invaluable tools to analyse and engineer biological processes. While temperature-sensitive alleles are frequently used as conditional mutants in microorganisms, they are usually difficult to identify in multicellular species. Here we present a versatile and transferable, genetically stable syst...

متن کامل

Ruminal Protein Degradation and Estimation of Rumen Microbial Protein Production

Animal agricultural production systems are major sources of nonpoint pollution affecting quality of water sources. Nitrogen has been identified as the foremost source of nonpoint water pollution  and the potential negative impacts of N have become an area of public concern. protein degradation from feed ingredients is an important factorinfluencing AA supply to the duodenum. Ruminal proteolysis...

متن کامل

Ruminal Protein Degradation and Estimation of Rumen Microbial Protein Production

Animal agricultural production systems are major sources of nonpoint pollution affecting quality of water sources. Nitrogen has been identified as the foremost source of nonpoint water pollution  and the potential negative impacts of N have become an area of public concern. protein degradation from feed ingredients is an important factorinfluencing AA supply to the duodenum. Ruminal proteolysis...

متن کامل

Azo-Containing Polymers with Degradation On-Demand Feature

Molecules comprising aliphatic azo moieties are widely used as radical polymerization initiators, but only few studies have explored their usefulness as stimuliresponsive motifs in macromolecular constructs. The controlled degradation of azo-containing polymers has indeed remained largely unexplored. Here we present the syntheses of linear azocontaining polyamides and polyurethanes and report o...

متن کامل

Evaluating the Effect of Protein A Signal Peptide on Extracellular Expression of Recombinant Hirudin in E.Coli

Abstract         Background and Objective: Hirudin is an anticoagulant polypeptide secreted from the salivary glands of leeches. Recombinant hirudin is a strong anticoagulant agent in arterial and venous thrombosis. The aim of this study was to evaluate the effect of inserting protein A signal peptide sequence of pEZZ18 plasmid on expression and secretion...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Nature Methods

سال: 2020

ISSN: 1548-7091,1548-7105

DOI: 10.1038/s41592-020-0952-3